| Fastest Heat - 5 | |||||
| Lane | Comp.No. | Name | YoB | Club | Time |
| 0 | 40 | Phoebe Arbuckle | 08 | Warrender Ba | : . |
| 1 | 42 | Hollie Wilson | 08 | Co Leeds | : . |
| 2 | 44 | Lucy Fox | 06 | Wycombe Dist | : . |
| 3 | 46 | Lucie Hanquet | 03 | Lboro Uni | : . |
| 4 | 48 | Amelie Blocksidge | 09 | Co Salford | : . |
| 5 | 47 | Fleur Lewis | 03 | Lboro Uni | : . |
| 6 | 45 | Michaella Glenister | 02 | UniOfStirl | : . |
| 7 | 43 | Ella Dyson | 04 | Wycombe Dist | : . |
| 8 | 41 | Amber Keegan | 97 | Co Sheffield | : . |
| 9 | 39 | Ava Cook | 08 | Co Sheffield | : . |