| Heat Number - 1 | ||||||
| Lane | Comp.No. | Name | YoB | Cat | Club | Time |
| 2 | 1 | Tegan Davidson | 06 | S6 | UoAPS | 56.30 |
| 3 | 2 | Ellie Challis | 04 | S3 | Clacton SC | 54.90 |
| 4 | 3 | Claire Connon | 85 | S4 | Co Cambridge | 52.62 |
| 5 | 4 | Rose Webb | 11 | S6 | Northampton | 49.94 |
| 6 | 5 | Maisie Catt | 09 | S7 | RichmondDale | 45.76 |
| 7 | 6 | Tully Kearney | 97 | S5 | Lboro Uni | 41.80 |