| Heat Number - 1 | ||||||
| Lane | Comp.No. | Name | AaD | Cat | Club | Time |
| 0 | 2 | Charlotte Hyde | 21 | S7 | Enfield Sq | 5:45.58 |
| 1 | 4 | Abigail Wickens | 16 | S8 | Flitwick | 5:37.88 |
| 2 | 6 | Isabella Haynes | 15 | S9 | S Bournem'th | 5:27.42 |
| 3 | 8 | Meghan Willis | 16 | S10 | Torfaen D | 5:16.17 |
| 4 | 10 | Amber Haycock | 16 | S10 | Northampton | 5:03.80 |
| 5 | 9 | Rebecca Lewis | 20 | S10 | RCT Squad | 5:14.47 |
| 6 | 7 | Astrid Carroll | 14 | S12 | Bo Kirklees | 5:17.82 |
| 7 | 5 | Lily Rice | 19 | S8 | Pembs Cty | 5:27.78 |
| 8 | 3 | Eliza Humphrey | 18 | S11 | Northampton | 5:45.44 |
| 9 | 1 | Fern Sneddon | 17 | S6 | RTW Monson | 6:06.50 |