| Final | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 1 | Zichen Liu | 92 | Derventio | 26.91 |
| 2 | 2 | James Lovatt | 89 | Derventio | 26.83 |
| 3 | 3 | Scott Hathaway | 88 | Loughboro Un | 26.78 |
| 4 | 4 | Liam Tancock | 85 | Loughboro Un | 25.07 |
| 5 | 5 | Luke Wood | 91 | Loughboro Un | 25.95 |
| 6 | 6 | Craig McNally | 92 | Warrender Ba | 26.80 |
| 7 | 7 | Calum Jarvis | 92 | Bath Univ | 26.90 |
| 8 | 8 | Charlie Boldison | 92 | Barnet Copt | 26.94 |